Search results for " Legionella pneumophila"

showing 3 items of 3 documents

Control and prevention measures for legionellosis in hospitals: A cross-sectional survey in Italy

2018

Abstract Risk assessment, environmental monitoring, and the disinfection of water systems are the key elements in preventing legionellosis risk. The Italian Study Group of Hospital Hygiene of the Italian Society of Hygiene, Preventive Medicine, and Public Health and the Italian Multidisciplinary Society for the Prevention of Health Care-Associated Infections carried out a national cross-sectional survey to investigate the measures taken to prevent and control legionellosis in Italian hospitals. A multiple-choice questionnaire was developed, comprising 71 questions regarding hospital location, general characteristics, clinical and environmental surveillance, and control and preventive measur…

0301 basic medicinemedicine.medical_specialtyCross-sectional studyLegionellamedia_common.quotation_subject030106 microbiologyControl measuresBiochemistryControl measures; Hospital; Legionellosis; National survey; Prevention; Biochemistry; 2300Legionella pneumophila03 medical and health sciencesHospital0302 clinical medicineControl measures; Hospital; Legionellosis; National survey; Prevention; Cross Infection; Cross-Sectional Studies; Disinfection; Humans; Infection Control; Italy; Legionella pneumophila; Legionellosis; Surveys and Questionnaires; Water Microbiology; Water SupplyHygieneWater SupplyLegionellosiSurveys and Questionnairesmedicinecontrol measures; hospital; legionellosis; national survey; preventionControl measures Hospital Legionellosis National survey Prevention Biochemistry 2300Humans030212 general & internal medicineSeroconversionGeneral Environmental SciencePreventive healthcaremedia_commonResponse rate (survey)National surveyCross InfectionInfection ControlLegionellosisbiology2300business.industryPublic healthPreventionControl measurebiology.organism_classificationDisinfectionCross-Sectional StudiesItalyEmergency medicinebusinessRisk assessmentWater Microbiology
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Single tube real time PCR for detection of Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella pneumophila from …

2012

We designed a multiplex real time PCR for rapid, sensitive and specific detection of Streptococcus pneumoniae, Legionella pneumophila, Chlamydophila pneumoniae and Mycoplasma pneumoniae. The study cases consisted of 129 patients with community acquired pneumonia (CAP). Bacteriological techniques were implemented for detection of the cultivable organisms. DNA were extracted from sputa, throat swabs, bronchoalveolar lavages and tracheal aspirates and used as templates in real time PCR. The primers and probes were designed for cbpA (S. pneumoniae), p1adhesin (M. pneumoniae), mip (L. pneumophila) and ompA (C. pneumoniae). After optimization of real time PCR for every organism, the experiments w…

Settore MED/07 - Microbiologia E Microbiologia ClinicaMycoplasma pneumoniaemedicine.disease_causeReal-Time Polymerase Chain ReactionLegionella pneumophilaSensitivity and SpecificityMicrobiologyLegionella pneumophilaCommunity-acquired pneumoniacommunity acquired pneumonia CAP real time PCR Streptococcus pneumonia Legionella pneumophila Chlamydophila pneumonia Mycoplasma pneumoniaeStreptococcus pneumoniaeMultiplex polymerase chain reactionmedicinePneumonia BacterialHumansMultiplexGeneral Immunology and MicrobiologybiologyBacteriaGeneral MedicineChlamydophila pneumoniaebiology.organism_classificationmedicine.diseaseVirologyrespiratory tract diseasesMycoplasma pneumoniaeCommunity-Acquired InfectionsReal-time polymerase chain reactionStreptococcus pneumoniaeChlamydophila pneumoniaeMultiplex Polymerase Chain ReactionActa microbiologica et immunologica Hungarica
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Serological and molecular identification of Legionella spp. isolated from water and surrounding air samples in Italian healthcare facilities

2016

Abstract Background Legionella is an intracellular microorganism living in natural and artificial aquatic environments. Although its transmission to humans is linked to the inhalation of contaminated aerosols, there is no validated air sampling method for the control and prevention of the disease. The aim of the present study was to provide more information on the distribution of Legionella spp. in indoor environments and to determine whether the same Legionella strains are isolated from air and water samples. Methods Ten healthcare facilities located in seven regions of Italy were enrolled. The serological typing of Legionella spp. from water samples and the surrounding air by active and p…

microbialSerotypesequence analysisLegionellaColony Count Microbialair microbiologyLegionelladna010501 environmental sciences01 natural sciencesLegionella pneumophilaBiochemistrySerologyMicrobiologySerological typing03 medical and health sciences0302 clinical medicinehealth facilitiesBioaerosol; Legionella; Molecular investigation; Serological typingEnvironmental Science(all)italy030212 general & internal medicineTypingcolony count0105 earth and related environmental sciencesGeneral Environmental ScienceMolecular identificationBioaerosolMolecular investigation2300bioaerosol; legionella; molecular investigation; serological typing; bacterial proteins; colony count microbial; drinking water; health facilities; italy; legionella pneumophila; sequence analysis dna; air microbiology; biochemistry;biologydrinking waterlegionella pneumophilaBioaerosol; Legionella; Molecular investigation; Serological typing.Bioaerosol Legionella Molecular investigation Serological typingSequence Analysis DNASequence typesbacterial infections and mycosesbiology.organism_classificationbacterial proteinsBioaerosol; Legionella; Molecular investigation; Serological typing; 2300; BiochemistryBioaerosolEnvironmental Research
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